National Referral Laboratory

Introduction

Grape is one of the important fresh fruit being exported to European Union countries besides the countries of Middle East and the Far East. Pesticide residue in the grapes is one of the quality parameters before the importers in importing countries to accept the produce. In 2013-04, the Indian grapes exported to European Union countries faced a complete ban due to presence of pesticide residues above the prescribed EU-MRL level. As a corrective action, EU authority has advised India to establish a traceability monitoring system of control of pesticide residues in grapes for further initiation of grape export to EU. As a repercussion to this, APEDA, Ministry of Commerce recognized ICAR-NRC for Grapes as the National Referral Laboratory (NRL) to establish traceability system for control pesticide residues in table grapes for export. Due to this centre’s dedicated services as National Referral Laboratory, the export of table grapes to EU restarted in 2004-05 and it improved significantly since last year with zero rapid alerts due to MRL exceedance. At domestic level, the MRL non-compliance of fresh grapes for export to EU countries has reduced from 24% in 2004 to around 5-7% in the recent years. Thus, there is increase in MRL compliance by around 17% due to the actives played by the ICAR-NRC Grapes as the National Referral Laboratory. This effect has also created a positive influence on the total export of fresh grapes from India, which has been increased from INR 105.89 crores in 2003-2004 just before implementation of the residue monitoring system to the current value of INR 2146.22 crores in 2017-18. Further, the official control of pesticide residues in grape has been extended to other export markets other than EU such as Russia, China, GCC, Indonesia, Canada etc. in the same line. This residue monitoring in export grapes has created awareness among domestic market as well and the centre initiated a “zero residue grape” program for domestic market since 2017-18 for better price realization for growers. As an overall consequence of this, the export has increased significantly over several years and the growers got a better price realization for their produce both at export and domestic market. On successful implementation of Residue Monitoring Plan in grape, the model was extended by APEDA to all other fruits and vegetables for monitoring pesticide residues and in peanut and peanut products for monitoring aflatoxins with ICAR-NRCG being the National referral Laboratory.

Objectives

Scope

All grape farmers, farms & plots intending to produce grapes for export to the European Union and Non-EU, exporters, APEDA recognized pack houses, APEDA recognized laboratories authorized for sampling, analysis and grading of grapes and personnel engaged by them, ICAR-NRC Grapes, Central Insecticide Board, DMI, respective State Government departments of Agriculture/Horticulture, Indian Council of Agriculture Research (ICAR) and any other agencies/ stakeholders including extension providers in any form to the farmers, producers and exporters cultivating crops for exports shall get covered under this

Responsibilities of the NRL

Accreditation/ Recognition to NRL:

ISO/IEC 17025: 2005 Accreditation:

NRL, ICAR-NRC for Grapes is accredited for testing of pesticide residues in different fruits and vegetables ad aflatoxins in peanut and peanut products by NABL. The scope of accreditation includes 223 pesticides in fruits and vegetables and 4 aflatoxins (B1,B2,G1,G2) in peanut and peanut products. This is highest scope for pesticides among any ICAR institutes

ISO/IEC 17043: 2010 Accreditation:

NRL, ICAR-NRC for Grapes is accredited “Proficiency Testing Provider” for pesticide residues in fruits and vegetables and aflatoxins in peanut and peanut products. This is the only institute in ICAR system having this accreditation.

Recognized as National Reference Laboratory by FSSAI:

NRL, ICAR-NRCG is being recognized as the National reference Laboratory 9apex laboratory in the country) for pesticide residues and aflatoxins in all matrices by Food Safety and Standard Authority of India.

Recognized as National Referral Laboratory by ICAR:

ICAR recognized this lab as the National referral Laboratory of pesticide residues and food safety in fruits. Accordingly, the mandate of institute has been revised.

Infrastructure facilities

1. High end equipment like LCMSMS, GCMSMS, ICPMS

SN. No.Name ( Model / type / year of make)
1.LC-MS/MS, (API 4000 QTrap, AB Sciex, USA)
2.LC-MS/MS (5500 Q.Trap, AB Sciex, USA)
3.LC-MS/MS (API 2000 Q.Trap, AB Sciex, USA)
4.LC -MS/MS-APGC-MS/MS (Waters Xevo-TQS, USA)
5.UPLC- QToF- MS (Waters Synapt G2-Q-TOF, USA)
6.UHPLC-Q Exactive Orbitrap (Thermo Scientific, USA)
7.GC-MS/MS (QqQ, 7000B), Agilent Technologies, USA
8.GC-MS/MS-TQ-8040 (Shimadzu, Japan)
9.MDGC-MS-Single Quadrapole QP-2010 (Shimadzu, Japan)
10.GC X GC – ToF-MS (Leco Corporation, USA)
11.ICP-LC-MS (Agilent Technologies, USA)

2. Specialized Equipment like HPLC, GC etc.

SN. No.Name ( Model / type / year of make)
1.UPLC-FLR Detector, Waters Corporation, USA
2.UHPLC-Dionex ultimate 3000 Waters with DAD Detector
3.UPLC- TUV Detector, Waters Corporation, USA

3. General Equipment

SN. No.Name ( Model / type / year of make)
1.UV-Spectrophotometer- Shimadzu UV-1800
2.Accelerator Solvent Extractor (Dionex - 350)
3.Genevac Rocket Synergy Evaporation (Thermo Scientific)
4.Forced convection laboratory incubator (Esco- Isotherm)
5.Bench top freeze dryer system (Labconco)
6.Bench top freeze dryer system (Virtis)
7.Rotary Evaporator (Heidolph)
8.Spray Dryer ( Techno search)
9.Low volume Concentrator, Turbovap LV Caliper
10.Walk-in Cold Storage (-200C and 00C), Blue star
11.Vertical Deep Freezer, FS-345 (Vest frost blue star ltd.)
12.Refrigerator, -20o C Deep freezer Samsung
13.Refrigerated centrifuge, Kubota 6500
14.Cryocube-F570 -80o C Deep freezer (Thermo search)
15.Orbital Shaker, Scigenics Orbitek
16.Precision Electronic Balance , Adair Dutt ADGR 220
17.Analytical balance, Adair Dutt AJ – 420E-A-Vibra
18.High Micro centrifuge, D-Lab D2012 plus
19.Microcentrifuge, Biofuge Pico Heraeus

Annexure 5: List of chemicals with CIB & RC label claim for use in grapes
Annexure 9: List of Agrochemicals to be monitored








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